Dunaliella Cell Counting

Dunaliella salina Cell Counts

Objective: Learn how to count cells using a hemocytometer.                                                                    

Procedure:

• Separate Dunaliella salina cells from any impurities in the stock sample                          
  • Centrifuge the sample for 6 mins in a 4˚ C room                                               
  • Pipette supernatant out

• Load hemocytometer (Tutorial)
• Set hemocytometer on microscope stage
• Zoom in to 40x magnification                                   
• Count cells

Counting Cells

To count the Dunaliella salina cells we had to use a hemocytometer that has four different sections. We counted the cells section by section until we found out the total amount of cells displayed. Afterwards, we used a specific mathematical equation to figure out the amount of cells per milliliter.

          Equation:               (average number of cells per quadrant)  x  (1000 mm³)  =  cells

                                                                    0.1 mm³                                                 (1 cm³)               mL

         Hemocytometer: