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About ISB
Baliga
Group
High
School Interns
Halobacterium
Our Work
Making Cultures
Streaking Plates
Salinity Lab
Halo Growth with Incubator
Halo Growth without Incubator
Harvesting Membranes
Halo
Crystals
Microbiology
Skills Pt. 1
Microbiology
Skills Pt. 2
Our Mentors
Institute for
Systems Biology
1441 North 34th Street
Seattle, WA 98103-8904
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Streaking Plates
Purpose:
To recover strains that are frozen for later experiments.
Protocol:
Take strains out of freezer(at -80 degrees C). Gently dig
into the frozen strains using pointed side of streaking instrument.
Streak strains on CM agar petrie dishes (see diagram below). Put petrie
dishes in plastic bag, upsidedown to prevent condensation from dripping
onto the strains. Store in incubator at 37 degrees C for 10 days.
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