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Halobacterium
Our Work
Making Cultures
Streaking Plates
Salinity Lab
Halo Growth with Incubator
Halo Growth without Incubator
Harvesting Membranes
Halo
Crystals
Microbiology
Skills Pt. 1
Microbiology
Skills Pt. 2
Our Mentors
Institute for
Systems Biology
1441 North 34th Street
Seattle, WA 98103-8904
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Life in an Extreme Environment Kit (Halo Crystals)
Purpose:
We got this experiment in a kit from a company and wanted to try it out
to see if it would be a good experiment for us to try to modify into a
kit of our own. We also wanted to see if making cultures from
halo in a salt crystal would work.
Protocol:
Filled 10 tubes with 5ml of liquid media. Poured salt crystals
onto napkin and put 3 crystals into each tube. For tubes not in shake
incubator shake the tubes daily for 20 minutes to aerate them. At
37 degrees C it should take 1-2 weeks to for the culture to turn pink.
At room temperature it should take two weekes or longer. In
the shaker incubator we had 1 negative control, 3 we put crystals in
using sterile tongues, and 3 we used gloved hands. For the
samples sitting on the lab bench, being shaken each day, we used outr
gloved hands to put in crystals to 3 tubes.
Results:
After 3 weeks of shaking the samples on the bench had no growth.
After 2 weeks in the incubator the majority of the samples were
lacking growth and the ones that had grown were barely noticeable.
Conclusion:
We discovered that using halo in salt crystal form is a faulty method
and doesn't yield good results. The tubes sitting on the bench
also might have not grown because of lack of sufficient oxygen getting
to the cells. |
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