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Baliga
Group
High
School Interns
Halobacterium
Our Work
Making Cultures
Streaking Plates
Salinity Lab
Halo Growth with Incubator
Halo Growth without Incubator
Harvesting Membranes
Halo
Crystals
Microbiology
Skills Pt. 1
Microbiology
Skills Pt. 2
Our Mentors
Institute for
Systems Biology
1441 North 34th Street
Seattle, WA 98103-8904
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Microbiology Skills Part 1
Purpose:
To test out a kit from a different company and see if it's something we
want to use in our curriculum. The kit explores how effective
different streaking methods are for growing colonies on plates.
Protocol:
We wiped down our work space with ethanol. We used a mixture
of disposable and reusable wire loops to streak CM agar plates in three
different ways. We dipped the loop in the culture and then
streaked, disposing of the plastic loops after every streak and flaming
the wire loop after every streak. After streaking, we allowed
the plates to sit for 20 minutes so the cells could adhere to the agar.
We inverted the plates and stored them in airtight bags for
28 days on the benchtop at room temperature.
Results:
After 28 days our plates looked like this...
The streaking worked. Even though it was difficult to tell
differences between the different options in a classroom environment
it'd be a good way to spark questions and learn. However, it took
a very long time to grown without an incubator.
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