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About ISB
Baliga
Group
High
School Interns
Halobacterium
Our Work
Making Cultures
Streaking Plates
Salinity Lab
Halo Growth with Incubator
Halo Growth without Incubator
Harvesting Membranes
Halo
Crystals
Microbiology
Skills Pt. 1
Microbiology
Skills Pt. 2
Our Mentors
Institute for
Systems Biology
1441 North 34th Street
Seattle, WA 98103-8904
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Salinity Lab
Purpose:
To gain a basic understanding of how to run experiments in the lab and
to test the Salinity Lab to ensure that it would work in a high school
environment. Experiment's goal is to find how well Halobacterium
grow in different salt concentrations.
Protocol:
Find halo sample with OD600 closest to 0.6. Put halo samples into
shaker incubator for 48 hours at ~225rpm and 37 degrees C. After
removing them took the OD600 of all samples on the spectrophotometer.
Results:
Observations:
The data graphs show that for all samples the halo grew best at a
salinity level of 3.3M. The standard deviation of the 2.8M solution is
very large so there could ahve been something wrong with that part of
the experiment. The 2.8M solution in tube #1 is the outlier that
caused the large standard deviation.
Next:
The next step would be to conduct tests on just 2.8M to see if the data we got was a mistake or whether it's reproduceable.
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