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Baliga
Group
High
School Interns
Halobacterium
Our Work
Making Cultures
Streaking Plates
Salinity Lab
Halo Growth with Incubator
Halo Growth without Incubator
Harvesting Membranes
Halo
Crystals
Microbiology
Skills Pt. 1
Microbiology
Skills Pt. 2
Our Mentors
Institute for
Systems Biology
1441 North 34th Street
Seattle, WA 98103-8904
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Microbiology Skills Part 2
Purpose:
To evaluate a science curriculum kit from a company to see if there's anything from it that could be useful for our curriculum.
Protocol:
Making Lawns-
We used sterile technique throughout the experiment. We put 5
drops from provided dropper onto an agar plate. Then, using a
sterile spreader, we spread the drops evenly across the surface using
using a smooth, circular motions. We let the plate sit for 30
minutes so the cells could adhere to the agar. Then we incubated
them for 2.5 weeks at 37 degrees Celsius.
Serial Dilution-
We made a 4M solution of salt by combining 2.5 g of salt and enough
water to make the total volume 10ml. We added 18 drops of the
solution to 8 numbered tubes. Then we added 2 drops of halo
culture to tube 1 and mixed it in. Using a new pipet we added 2
drops from tube 1 to tube 2 and mixed well. We repeated that for
all 8 tubes. Using a new pipet and spreader for each plate we
spread 2 drops from tubes 2,4,6, and 8 to its corresponding plate.
We then incubated the plates for 2.5 weeks.
Results:
Making Lawns-
After 2.5 weeks our lawns looked like this...
Serial Dilution-
After 2.5 weeks our dilutions looked like this...
Starting from the upper left hand plate and going clockwise they are numbered 2,4,8, and 6.
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