Biofuels Project (Nazli and Jordan)
8:30 – Arrive, measure Chlamydomonas reinhardtii growth, form task lists for the day
9:00 – Make media, put reactors into autoclave
10:00 – Work on experiments for bioengineering curriculum and create student examples
12:00 – Lunch with the other interns
12:30 – Work with mentor on electroporation experiments
2:00 – Meeting with teachers about Bioengineering Curriculum
3:00 – Finish lab duties, pour plates, streak if needed
4:30 – Measure Chlamydomonas reinhardtii
5:00 – Leave ISB
Transcriptomics (Brooke and Myles)
8:30 am: arrive
8:45 am: measure OD values of bulch tubes in the lab
9:00 am: answer learning objective questions, read papers
9:30 am: start working on the computer project where we left off yesterday
10:30 am: meet with our mentor about problems with our program
11:00 am: after getting instruction, continue computer work with newfound confidence
12:30 pm: lunch with the other interns
1:00 pm: revise Allee effect protocol
2:00 pm: collect OD values for the 96-well plates in the lab
2:30 pm: continue work on the computer
4:00 pm: intern website planning meeting
5:00 pm: leave ISB
Systems Medicine (Chhy Chhy and Arwa)
8:30 am: Arrive, set up before Ambassadors come in
9:00 am: Greet ambassadors and begin activities
10:00 am: Listen to guest speakers, do some hands-on activities
12:00 pm: Lunch with other interns
12:30 pm: Continue working with Ambassadors
3:00 pm: Have a debrief meeting with our mentor and teachers about the day, clean up
4:00 pm: Work on assigned tasks like preps to lead activities for the Ambassadors
Proteomics (Elisa and Iman)
8:30 am: arrive, grab a cup of fruit tea in our cool ISB mugs, check the calendar for any new events throughout the day, and meet with mentor to discuss tasks
9:00 am: lab work with sample preparation of obesity samples using s-trap digestion, gel electrophoresis, etc. (one method usually requires the entire day); every other week we had a website planning meeting at 9:00, so we would start lab work around 10.
10:00 am: continue lab work; go to Hood-Price Lab Meeting; or go to a guest speaker’s talk
11:00 am: continue lab work; usually waiting for incubation or speed vac, so we researched proteomics/read papers
12:00 pm: make sure next steps of protocol are understood and the solutions needed are made
12:30 pm: lunch break with interns (many places nearby! Plus, the ritual app is amazing for dollar food fest).
1:00 pm: complete S-trap digestion, in solution digestion, gel electrophoresis. During waiting periods, start next steps of protocol or read papers (or scientific news!)
3:00 pm: once mass spec results started coming in, we booked conference rooms to discuss the results. Sometimes this took more than one hour depending on the topic of discussion.
4:00 pm : website planning meeting or planning ahead for the coming days
5:00/5:30 pm: leave ISB
Data Analysis (Sanjay)
8:45 am: Come into the office, get some tea, check-in with my mentor
9:00 am: Do some research on current algorithms used for clustering
10:00 am: Continue research on Adolescent Brain Cognitive Disorder(ABCD) datasets
11:00 am: Run fingerprint analysis on the fingerprints from ABCD data
12:00 pm: Let program run, go out for lunch with interns
1:00 pm: Try percent-variant sampling of fingerprint analysis to understand how my project can be scaled to larger datasets
2:00 pm: Take a ping pong break
2:10 pm: Run the percent sampling through R to visualize results
3:00 pm: Meet with my mentor, analyze the graphs and make decisions about how to move forward
4:00 pm: Website planning meeting with the other interns
5:00 pm: Skrrt