Wild type replicates begin to collapse after 3 transitions while the regulatory mutant grows across all transitions. Each line represents one of three replicate cultures. Filled circle symbols indicate the time-points and replicates that were sampled from WT and mutant for single cell gene expression analysis.
Three cell states were defined among single cells from each conditions, including state A (expression pattern present only during ST1 growth in wild type), state B (expression pattern present only during SR1 growth in wild type), and state C (expression pattern present during both SR1 and ST1 growth in wild type).
