Single Cell Analytics to Determine Robustness of a Model Microbial Community

May 1, 2017

Robustness of a model microbial community emerges from population structure among single cells of a clonal population

Robustness of a model microbial community emerges from population structure among single cells of a clonal population Thompson, A.W, Turkarslan S., Arens, C.E, López García de Lomana, A., Raman, A., Stahl, D.A., Baliga, N.S. Environmental Microbiology, 10.1111/1462-2920.13764

Wild type replicates begin to collapse after 3 transitions while the regulatory mutant grows across all transitions. Each line represents one of three replicate cultures. Filled circle symbols indicate the time-points and replicates that were sampled from WT and mutant for single cell gene expression analysis.
Three cell states were defined among single cells from each conditions, including state A (expression pattern present only during ST1 growth in wild type), state B (expression pattern present only during SR1 growth in wild type), and state C (expression pattern present during both SR1 and ST1 growth in wild type).

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dr Anne Thompson Portland State University https://www.pdx.edu/microbial-ecology/people