Dunaliella salina
Dunaliella salina is a halophillic eukaryote with two flagella. It needs strong light to thrive as it undergoes photosynthesis. Dunaliella grows best when exposed to a light intensity of 60 W/M² for 16 hours per day. They also needs a steady supply of air, so using a vented cap to allow air exchange is recommended. If you can use an incubator that shakes, more CO2 mixes with the Dunaliella causing the cultures to grow more rapidly. It takes about a week to increase the population to ten times the original. Although there is great variance between different strains of Dunaliella, most of them grow best at a pH of 8.2-8.7 and are able to survive at a pH from 7-9. They thrive at a temperature of 20-22º C, but room temperature is also acceptable. Do not put Dunaliella into freezers because they die from heat shock shortly after removal. Most Dunaliella grows well at a salinity of 3,000 - 10,000 ppm (parts per million). They should be inoculated into new cultures at a ratio of at least 1 mL culture to 250 mL medium. When healthy, Dunaliella are green and pear shaped.
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