1. Make and Auto clave 200 mL of Complete Media with Agar solutions then pour into a 100x 15-mm Petri dish.

2. Add 2 ml of Halobacterium to the agar plate and use about 15 beads to spread the halobacterium evenly across the plate by shaking it. Allow the cuture to incubate for 10 days

3. While using a spreader, wash the cells from the agar plate with 15mL of 1.0 mM MgSO4 containing 10ug/ml of Dnase I. Collect cell suspension in a large falcon tube (50mL)

4. Incubate cell suspension for 3 hours at 37 degrees Celsius. (No agitation)

5.Add cell suspension to centrifuge tubes, add 5% NaCl until the tube's final volumes are equal. Centrifuge cellls overnight at 60g in a swing bucket rotator.

6. Using a Pasteur pipet, recover the intact vesicles that are floating at the top and add them to a new centrifuge tube, add 5% NaCl until the tube's final volumes are equal. Centrifuge the cells overnight at 60g in a swing bucket rotator.

7. Repeat step 6 to create a total of three separations by centrifugation.

8. Recover all remaining intact gas vesicles by using a Pasteur Pipette and placing them in a beaker, keep at room temperature.