Untitled Document

Salinity Lab

THE PROCEDURE:

This lab was inspired by the Great Salt Lake because the salinity levels in the lake range, so by conducting the salinity lab one could compare the results to the Great Salt Lake and see where Halobacterium will have an optimal growth.
Complete Medium solutions were already prepared for the interns. The solution concentrations were: 4.5M, 4.0M, 3.5M, 3.0M, 2.5M, and 2.0M. 100 [mL] of Halobacterium were inoculated to each of the five concentrations and incubated for 48 hours in 37 degrees Celsius at 220 rpm. After the incubation the interns measured the cell density by using a spectrophotometer.
In result the interns came to the conclusion that Halobacterium lived best at optimal salt concentration of 3.3M and after that Halobacteriaum stayed constant.

THE REFLECTION:

By doing this experiment we didn’t only test what the optimal growth for Halobacterium was but got an understanding of different organisms being able to survive at different salinity levels. A body of salt water will have differences in salinity levels at different places, and therefore different organisms at different places.

	Our Research