TY - JOUR TI - Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. AU - Baliga, Nitin S. AU - Bonneau, Richard AU - Facciotti, Marc T. AU - Pan, Min AU - Glusman, Gustavo AU - Deutsch, Eric W. AU - Shannon, Paul AU - Chiu, Yulun AU - Weng, Rueyhung Sting AU - Gan, Rueichi Richie AU - Hung, Pingliang AU - Date, Shailesh V. AU - Marcotte, Edward AU - Hood, Leroy AU - Ng, Wailap Victor T2 - Genome research AB - We report the complete sequence of the 4,274,642-bp genome of Haloarcula marismortui, a halophilic archaeal isolate from the Dead Sea. The genome is organized into nine circular replicons of varying G+C compositions ranging from 54% to 62%. Comparison of the genome architectures of Halobacterium sp. NRC-1 and H. marismortui suggests a common ancestor for the two organisms and a genome of significantly reduced size in the former. Both of these halophilic archaea use the same strategy of high surface negative charge of folded proteins as means to circumvent the salting-out phenomenon in a hypersaline cytoplasm. A multitiered annotation approach, including primary sequence similarities, protein family signatures, structure prediction, and a protein function association network, has assigned putative functions for at least 58% of the 4242 predicted proteins, a far larger number than is usually achieved in most newly sequenced microorganisms. Among these assigned functions were genes encoding six opsins, 19 MCP and/or HAMP domain signal transducers, and an unusually large number of environmental response regulators-nearly five times as many as those encoded in Halobacterium sp. NRC-1--suggesting H. marismortui is significantly more physiologically capable of exploiting diverse environments. In comparing the physiologies of the two halophilic archaea, in addition to the expected extensive similarity, we discovered several differences in their metabolic strategies and physiological responses such as distinct pathways for arginine breakdown in each halophile. Finally, as expected from the larger genome, H. marismortui encodes many more functions and seems to have fewer nutritional requirements for survival than does Halobacterium sp. NRC-1. DA - 2004/11//undefined PY - 2004 DO - 10.1101/gr.2700304 VL - 14 IS - 11 SP - 2221 EP - 2234 J2 - Genome Res LA - eng SN - 1088-9051 1088-9051 KW - *Genome, Archaeal KW - Archaeal Proteins/*genetics/metabolism KW - Conserved Sequence/genetics KW - Haloarcula marismortui/*genetics/metabolism KW - Molecular Sequence Data KW - Oceans and Seas KW - Sequence Homology, Amino Acid KW - Software ER -