TY - JOUR TI - Genome sequence of Halobacterium species NRC-1. AU - Ng, W. V. AU - Kennedy, S. P. AU - Mahairas, G. G. AU - Berquist, B. AU - Pan, M. AU - Shukla, H. D. AU - Lasky, S. R. AU - Baliga, N. S. AU - Thorsson, V. AU - Sbrogna, J. AU - Swartzell, S. AU - Weir, D. AU - Hall, J. AU - Dahl, T. A. AU - Welti, R. AU - Goo, Y. A. AU - Leithauser, B. AU - Keller, K. AU - Cruz, R. AU - Danson, M. J. AU - Hough, D. W. AU - Maddocks, D. G. AU - Jablonski, P. E. AU - Krebs, M. P. AU - Angevine, C. M. AU - Dale, H. AU - Isenbarger, T. A. AU - Peck, R. F. AU - Pohlschroder, M. AU - Spudich, J. L. AU - Jung, K. W. AU - Alam, M. AU - Freitas, T. AU - Hou, S. AU - Daniels, C. J. AU - Dennis, P. P. AU - Omer, A. D. AU - Ebhardt, H. AU - Lowe, T. M. AU - Liang, P. AU - Riley, M. AU - Hood, L. AU - DasSarma, S. T2 - Proceedings of the National Academy of Sciences of the United States of America AB - We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can serve as an excellent model system among the archaea. DA - 2000/10/24/ PY - 2000 DO - 10.1073/pnas.190337797 VL - 97 IS - 22 SP - 12176 EP - 12181 J2 - Proc Natl Acad Sci U S A LA - eng SN - 0027-8424 0027-8424 KW - *Genome, Bacterial KW - Biological Evolution KW - Cell Membrane/metabolism KW - DNA Repair KW - DNA Replication KW - Energy Metabolism KW - Halobacterium/*genetics/metabolism KW - Lipid Bilayers KW - Molecular Sequence Data KW - Protein Biosynthesis KW - Recombination, Genetic KW - Signal Transduction KW - Transcription, Genetic ER -