{"id":165,"date":"2019-08-01T23:33:08","date_gmt":"2019-08-01T23:33:08","guid":{"rendered":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/?page_id=165"},"modified":"2019-10-28T04:52:53","modified_gmt":"2019-10-28T04:52:53","slug":"a-day-in-the-life","status":"publish","type":"page","link":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/a-day-in-the-life\/","title":{"rendered":"A Day in the Life"},"content":{"rendered":"\n<p><\/p>\n\n\n\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<h4 class=\"wp-block-heading\">Biofuels Project (Nazli and Jordan)<\/h4>\n\n\n\n<p>8:30 &#8211; Arrive, measure <em>Chlamydomonas reinhardtii <\/em>growth, form task lists for the day<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_4423-e1566411011105-768x1024.jpg\" alt=\"\" class=\"wp-image-603\" width=\"258\" height=\"342\" \/><\/figure><\/div>\n\n\n\n<p>9:00 &#8211; Make media, put reactors into autoclave<\/p>\n\n\n\n<p>10:00 &#8211; Work on experiments for bioengineering curriculum and create student examples<\/p>\n\n\n\n<p>12:00 &#8211; Lunch with the other interns<\/p>\n\n\n\n<p>12:30 &#8211; Work with mentor on electroporation experiments&nbsp;<\/p>\n\n\n\n<p>2:00 &#8211; Meeting with teachers about Bioengineering Curriculum<\/p>\n\n\n\n<p>3:00 &#8211; Finish lab duties, pour plates, streak if needed&nbsp;<\/p>\n\n\n\n<p>4:30 &#8211; Measure <em>Chlamydomonas reinhardtii&nbsp;<\/em><\/p>\n\n\n\n<p>5:00 &#8211; Leave ISB<br><\/p>\n\n\n\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<h4 class=\"wp-block-heading\">Transcriptomics (Brooke and Myles)<\/h4>\n\n\n\n<p>8:30 am: arrive<\/p>\n\n\n\n<p>8:45 am: measure OD values of bulch tubes in the lab<\/p>\n\n\n\n<p>9:00 am: answer learning objective questions, read papers<\/p>\n\n\n\n<p>9:30 am: start working on the computer project where we left off yesterday<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/53993F01-A043-4454-8BE6-0091A16DBD16-1024x768.jpg\" alt=\"\" class=\"wp-image-606\" width=\"324\" height=\"243\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/53993F01-A043-4454-8BE6-0091A16DBD16-1024x768.jpg 1024w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/53993F01-A043-4454-8BE6-0091A16DBD16-300x225.jpg 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/53993F01-A043-4454-8BE6-0091A16DBD16-768x576.jpg 768w\" sizes=\"auto, (max-width: 324px) 100vw, 324px\" \/><\/figure><\/div>\n\n\n\n<p>10:30 am: meet with our mentor about problems with our program<\/p>\n\n\n\n<p>11:00 am: after getting instruction, continue computer work with newfound confidence<\/p>\n\n\n\n<p>12:30 pm: lunch with the other interns<\/p>\n\n\n\n<p>1:00 pm: revise Allee effect protocol<\/p>\n\n\n\n<p>2:00 pm: collect OD values for the 96-well plates in the lab<\/p>\n\n\n\n<p>2:30 pm: continue work on the computer<\/p>\n\n\n\n<p>4:00 pm: intern website planning meeting<\/p>\n\n\n\n<p>5:00 pm: leave ISB<br><\/p>\n\n\n\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<h4 class=\"wp-block-heading\">Systems Medicine (Chhy Chhy and Arwa)<\/h4>\n\n\n\n<p>8:30 am: Arrive, set up before Ambassadors come in<\/p>\n\n\n\n<p>9:00 am: Greet ambassadors and begin activities<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/09\/STEAM-1-1024x684.jpg\" alt=\"\" class=\"wp-image-801\" width=\"327\" height=\"218\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/09\/STEAM-1-1024x684.jpg 1024w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/09\/STEAM-1-300x200.jpg 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/09\/STEAM-1-768x513.jpg 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/09\/STEAM-1-272x182.jpg 272w\" sizes=\"auto, (max-width: 327px) 100vw, 327px\" \/><\/figure><\/div>\n\n\n\n<p>10:00 am: Listen to guest speakers, do some hands-on activities<\/p>\n\n\n\n<p>12:00 pm: Lunch with other interns<\/p>\n\n\n\n<p>12:30 pm: Continue working with Ambassadors<\/p>\n\n\n\n<p>3:00 pm: Have a debrief meeting with our mentor and teachers about the day, clean up&nbsp;<\/p>\n\n\n\n<p>4:00 pm: Work on assigned tasks like preps to lead activities for the Ambassadors<\/p>\n\n\n\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<h4 class=\"wp-block-heading\">Proteomics (Elisa and Iman)<\/h4>\n\n\n\n<p>8:30 am: arrive, grab a cup of fruit tea in our cool ISB mugs, check the calendar for any new events throughout the day,  and meet with mentor to discuss tasks<\/p>\n\n\n\n<p>9:00 am: lab work with sample preparation of obesity samples using s-trap digestion, gel electrophoresis, etc. (one method usually requires the entire day); every other week we had a website planning meeting at 9:00, so we would start lab work around 10.&nbsp;<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_2072-768x1024.jpeg\" alt=\"\" class=\"wp-image-600\" width=\"237\" height=\"315\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_2072-768x1024.jpeg 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_2072-225x300.jpeg 225w\" sizes=\"auto, (max-width: 237px) 100vw, 237px\" \/><\/figure><\/div>\n\n\n\n<p>10:00 am: continue lab work; go to Hood-Price Lab Meeting; or go to a guest speaker&#8217;s talk&nbsp;<\/p>\n\n\n\n<p>11:00 am: continue lab work; usually waiting for incubation or speed vac, so we researched proteomics\/read papers&nbsp;<\/p>\n\n\n\n<p>12:00 pm: make sure next steps of protocol are understood and the solutions needed are made&nbsp;<\/p>\n\n\n\n<p>12:30 pm: lunch break with interns (many places nearby! Plus, the ritual app is amazing for dollar food fest).<\/p>\n\n\n\n<p>1:00 pm: complete S-trap digestion, in solution digestion, gel electrophoresis. During waiting periods, start next steps of protocol or read papers (or scientific news!)&nbsp;<\/p>\n\n\n\n<p>3:00 pm: once mass spec results started coming in, we booked conference rooms to discuss the results. Sometimes this took more than one hour depending on the topic of discussion.&nbsp;<\/p>\n\n\n\n<p>4:00 pm :  website planning meeting or planning ahead for the coming days<\/p>\n\n\n\n<p>5:00\/5:30 pm: leave ISB <\/p>\n\n\n\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<h4 class=\"wp-block-heading\">Data Analysis (Sanjay)<\/h4>\n\n\n\n<p>8:45 am: Come into the office, get some tea, check-in with my mentor<\/p>\n\n\n\n<p>9:00 am: Do some research on current algorithms used for clustering<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_1856-768x1024.jpg\" alt=\"\" class=\"wp-image-616\" width=\"253\" height=\"337\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_1856-768x1024.jpg 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_1856-225x300.jpg 225w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-content\/uploads\/sites\/7\/2019\/08\/IMG_1856.jpg 1182w\" sizes=\"auto, (max-width: 253px) 100vw, 253px\" \/><\/figure><\/div>\n\n\n\n<p>10:00 am: Continue research on Adolescent Brain Cognitive Disorder(ABCD) datasets<\/p>\n\n\n\n<p>11:00 am: Run fingerprint analysis on the fingerprints from ABCD data<\/p>\n\n\n\n<p>12:00 pm: Let program run, go out for lunch with interns<\/p>\n\n\n\n<p>1:00 pm: Try percent-variant sampling of fingerprint analysis to understand how my project can be scaled to larger datasets<\/p>\n\n\n\n<p>2:00 pm: Take a ping pong break<\/p>\n\n\n\n<p>2:10 pm: Run the percent sampling through R to visualize results<\/p>\n\n\n\n<p>3:00 pm: Meet with my mentor, analyze the graphs and make decisions about how to move forward<\/p>\n\n\n\n<p>4:00 pm: Website planning meeting with the other interns<\/p>\n\n\n\n<p>5:00 pm: Skrrt<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biofuels Project (Nazli and Jordan) 8:30 &#8211; Arrive, measure Chlamydomonas reinhardtii growth, form task lists for the day 9:00 &#8211; Make media, put reactors into autoclave 10:00 &#8211; Work on experiments for bioengineering curriculum and create student examples 12:00 &#8211; Lunch with the other interns 12:30 &#8211; Work with mentor on electroporation experiments&nbsp; 2:00 &#8211; [&hellip;]<\/p>\n","protected":false},"author":51,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-165","page","type-page","status-publish","hentry","post"],"_links":{"self":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/pages\/165","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/users\/51"}],"replies":[{"embeddable":true,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/comments?post=165"}],"version-history":[{"count":8,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/pages\/165\/revisions"}],"predecessor-version":[{"id":922,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/pages\/165\/revisions\/922"}],"wp:attachment":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2019\/wp-json\/wp\/v2\/media?parent=165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}