{"id":526,"date":"2018-08-08T17:59:23","date_gmt":"2018-08-08T17:59:23","guid":{"rendered":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/?page_id=526"},"modified":"2018-08-24T23:44:08","modified_gmt":"2018-08-24T23:44:08","slug":"lyme-project","status":"publish","type":"page","link":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/lyme-project\/","title":{"rendered":"Lyme Disease"},"content":{"rendered":"<h4><a href=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/emily-huynh\/\">Emily Huynh<\/a>\u00a0&amp;\u00a0<a href=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/high-school-interns-2018\/shannon-chau\/\">Shannon Chau<\/a><\/h4>\n<h1><div id=\"metaslider-id-1453\" style=\"max-width: 900px;\" class=\"ml-slider-3-102-0 metaslider metaslider-flex metaslider-1453 ml-slider has-dots-nav ms-theme-default\" role=\"region\" aria-label=\"Lyme\" data-height=\"651\" data-width=\"900\">\n    <div id=\"metaslider_container_1453\">\n        <div id=\"metaslider_1453\">\n            <ul class='slides'>\n                <li style=\"display: block; width: 100%;\" class=\"slide-1456 ms-image \" aria-roledescription=\"slide\" data-date=\"2018-08-24 16:52:00\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/DSC_5034-900x651.jpg\" height=\"651\" width=\"900\" alt=\"\" class=\"slider-1453 slide-1456 msDefaultImage\" title=\"DSC_5034\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-1461 ms-image \" aria-roledescription=\"slide\" data-date=\"2018-08-24 16:55:20\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/DSC_5424-2-1-900x651.jpg\" height=\"651\" width=\"900\" alt=\"\" class=\"slider-1453 slide-1461 msDefaultImage\" title=\"DSC_5424 (2)\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-1457 ms-image \" aria-roledescription=\"slide\" data-date=\"2018-08-24 16:52:07\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/2018-08-08-10.20.33-1-900x651.jpg\" height=\"651\" width=\"900\" alt=\"\" class=\"slider-1453 slide-1457 msDefaultImage\" title=\"Processed with VSCO with  preset\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-1458 ms-image \" aria-roledescription=\"slide\" data-date=\"2018-08-24 16:52:11\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/DSC_4981-900x651.jpg\" height=\"651\" width=\"900\" alt=\"\" class=\"slider-1453 slide-1458 msDefaultImage\" title=\"DSC_4981\" \/><div class=\"caption-wrap\"><div class=\"caption\">Partners Emily and Shannon<\/div><\/div><\/li>\n            <\/ul>\n        <\/div>\n        \n    <\/div>\n<\/div><\/h1>\n<h3><strong>Overview of Lyme Disease and the Lyme Project<\/strong><\/h3>\n<figure id=\"attachment_1057\" aria-describedby=\"caption-attachment-1057\" style=\"width: 300px\" class=\"wp-caption alignright\"><a href=\"https:\/\/www.cdc.gov\/lyme\/stats\/maps.html\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1057 size-medium\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/2016-dot-map-300x212.jpg\" alt=\"\" width=\"300\" height=\"212\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/2016-dot-map-300x212.jpg 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/2016-dot-map.jpg 500w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><figcaption id=\"caption-attachment-1057\" class=\"wp-caption-text\">2016: Cases of Lyme disease in the US<\/figcaption><\/figure>\n<p><span style=\"font-weight: 400\">Lyme disease is a multi-organ illness that is transmitted to humans via ticks. Ticks act as a vector for the disease-causing spirochete <\/span><i><span style=\"font-weight: 400\">Borrelia<\/span><\/i> <i><span style=\"font-weight: 400\">burgdo<\/span><\/i><i>feri.<\/i> Lyme disease was traditionally prevalent in the Northeast region, but is now found in all fifty states. Cases have risen to over 300,000 each year (Center for Infectious Disease Control and Prevention). To this end, Lyme disease is now the most common vector-borne illness, and it ranks number six in most commonly reported infectious diseases. This rapid increase in Lyme disease cases poses a concern for both researchers and public health overall.<\/p>\n<p><span style=\"font-weight: 400\">Signs and symptoms of Lyme disease include a signature eyrthem\u00a0<\/span><span style=\"font-weight: 400\">a migrans rash (a red, bullseye-shaped mark), fever, chills, and headache. Oral antibiotics taken in early states of acute Lyme disease usually help patients recover fully. However, chronic lyme disease, also known as Post-Treatment Lyme Disease Syndrome (PTLDS), ca<\/span>n occur. This is a condition in which the patient has symptoms long after treatment has ended. The causes and long-term effects of PTLDS are still unknown. In addition, diagnosis of PTLDS is surrounded by controversy due to a lack of consensus and misconceptions within the medical community.<\/p>\n<p><span style=\"font-weight: 400\">Thus, to address the rapid s<\/span><span style=\"font-weight: 400\">pread of Lyme disease and deficient knowledge surrounding its chronic form, researchers at ISB are studying the con<\/span>dition through many approaches including proteomic analysis.<\/p>\n<h3><strong>Proteomic\u00a0 Training\/Skills<\/strong><\/h3>\n<p><span style=\"font-weight: 400\">In order to be able to execute the lab work necessary for their project, Emily and Shannon spent a portion of the s<\/span>ummer adding to their lab skill set. They learned <strong>BCA and Micro-BCA Protein Assays\u00a0<\/strong> used for protein quantification. These techniques are relatively quick and easy for providing more information on the sample at hand.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1091 aligncenter\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/Capture-1-300x68.png\" alt=\"\" width=\"507\" height=\"115\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/Capture-1-300x68.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/Capture-1-760x173.png 760w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/Capture-1.png 765w\" sizes=\"auto, (max-width: 507px) 100vw, 507px\" \/><\/p>\n<p><span style=\"font-weight: 400\">An<\/span><span style=\"font-weight: 400\">\u00a0example of one of the B<\/span><span style=\"font-weight: 400\">CA protein assays that provided<\/span>\u00a0information for the S-Trap protocol.<\/p>\n<p><span style=\"font-weight: 400\">Another useful method that<\/span>\u00a0Emily and Shannon learned was <strong>gel electrophoresis<\/strong>. Initially, the two were taught how to run a gel for DNA. As their project developed, they learned how to apply the same principles to running a gel for proteins. This was accompanied with learning how to view the protein bands through silver staining.<\/p>\n<p><span style=\"font-weight: 400\">As a result of their constant work w<\/span>ith protein samples, Emily and Shannon had to quickly learn the proper way of <strong>handling proteins<\/strong>. In order to avoid protein degradation, proteins had to be properly stored at -80\u00b0C for long periods of time, -20\u00b0C for short periods of time,\u00a0and on wet or dry ice while executing work.<\/p>\n<h3><strong>S-Trap Protocol (Main Focus)<\/strong><\/h3>\n<h5><b style=\"font-size: 16px\">Optimization of Sample Preparation in Proteomic Analyses Using Suspension Trap Digestion<\/b><\/h5>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1374 aligncenter\" style=\"font-size: 16px\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/flow-chart-300x166.png\" alt=\"\" width=\"472\" height=\"261\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/flow-chart-300x166.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/flow-chart-768x425.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/flow-chart.png 950w\" sizes=\"auto, (max-width: 472px) 100vw, 472px\" \/><\/h3>\n<p><span style=\"font-weight: 400\">One of the essential parts of studying proteomics revolves around sample preparation, which is used to digest proteins down to their peptides. Research scientists can spend significant amount time and money preparing hundreds of samples at a time. One of the current ways that ISB prepares their protein samples for mass spectrometry is referred to as \u201ctraditional digestion.\u201d Traditional digestion is an in-solution digestion that involves a variety of steps \u2014 most notably, an overnight incubation period that can hinder lab productivity. To streamline<\/span>\u00a0the process, Emily and Shannon adjusted and evaluated a more efficient protocol using the suspension trap method, based on the S-Trap \u2122 column (a product from the biotechnology company, ProtiFi, LLC). The protocol that they created can be used to expedite sample preparation of Lyme disease patients&#8217; plasma samples.<\/p>\n<p>Lyme disease patient samples are often processed as depleted plasma. Unlike tissue samples (for which the original protocol was designed), depleted plasma samples lack cellular material that needs to be broken down. In addition, depleted samples have a much higher volume and lower protein concentration than pure plasma.<\/p>\n<p><span style=\"font-weight: 400\">Therefore, they considered the following obstacles when adjusting the protocol:\u00a0<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Side effects due to overuse of strong chemicals for digestion<\/span><\/li>\n<li style=\"font-weight: 400\">The low protein concentration<\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Incomplete protein digestion\/protein loss<\/span><\/li>\n<\/ol>\n<p>To account for these factors, Emily and Shannon tested multiple areas for revision. For one, they reduced reagent concentrations to avoid over use of the chemicals. They also tested multiple binding buffer ratios to evaluate how little they could add without compromising protein retention.<\/p>\n<p>Later, Shannon and Emily utilized the proteomic skills of SDS-PAGE gel electrophoresis and silver staining to qualitatively analyze the S-Trap column\u2019s protein-binding power&#8211;and therefore its ability to decrease protein loss in comparison to traditional digestion.\u00a0They\u00a0ran gels on the flowthrough from the binding, washing, and trypsin digestion steps of the protocol, as well as the final elution and the traditional digestion. Then they noted how many proteins would have been lost as flowthrough in each step (had the flowthroughs been disposed) by comparing them to the final S-Trap eluted proteins and the traditional digestion proteins.<\/p>\n<figure id=\"attachment_1317\" aria-describedby=\"caption-attachment-1317\" style=\"width: 267px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1317\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.17.2018-300x225.png\" alt=\"\" width=\"267\" height=\"200\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.17.2018-300x225.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.17.2018-768x576.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.17.2018.png 771w\" sizes=\"auto, (max-width: 267px) 100vw, 267px\" \/><figcaption id=\"caption-attachment-1317\" class=\"wp-caption-text\">The SDS-PAGE gel displaying protein bands from a depleted plasma sample used in 1:1 S-Trap, 1:2 S-Trap, and the original plasma.<\/figcaption><\/figure>\n<figure id=\"attachment_1316\" aria-describedby=\"caption-attachment-1316\" style=\"width: 280px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1316\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.15.2018-gel-300x229.png\" alt=\"\" width=\"280\" height=\"214\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.15.2018-gel-300x229.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.15.2018-gel-768x585.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/8.15.2018-gel.png 803w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><figcaption id=\"caption-attachment-1316\" class=\"wp-caption-text\">The SDS-PAGE gel displaying protein bands from an original plasma sample, traditional digestion, 1:1 S-Trap, and 1:7 S-Trap digestion.<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>The SDS-PAGE on the left was used as an initial, qualitative evaluation method for the S-Trap. This gel has &#8220;blank&#8221; lanes in lanes x-x, which showed that protein loss was minimal in all of the flowthrough steps except for the addition of digestion buffer (labeled &#8220;Trypsin 1:1&#8221; and &#8220;Trypsin 1:7&#8221; in the gel lanes). Knowing this, Emily and Shannon repeated the experiment with a few adjustments.<\/p>\n<p>First, the higher binding buffer to sample volume ratio was decreased to a 1:2, rather than 1:7, since the 1:7 setup did not lose significant amounts of peptides.\u00a0Second, all flowthrough was disposed of except for that of the binding step (named &#8220;Binding FT&#8221;) because the experimenters wanted to continue testing different binding buffer ratios. Finally, Shannon and Emily decided to use a shorter centrifugation for the digestion buffer and to return all digestion buffer flowthrough to the top of the column. This was in attempt to reduce the peptides lost in the digestion buffer step prior to incubation. All of these changes proved to be successful once they were loaded into the second gel on the right.<\/p>\n<p>From there, the second trial&#8217;s design was repeated on depleted plasma samples (rather than original plasma samples) that were loaded into a mass spectrometer for a quantitative analysis of peptide retention.<\/p>\n<figure id=\"attachment_1393\" aria-describedby=\"caption-attachment-1393\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1393 size-medium\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-2-300x178.png\" alt=\"\" width=\"300\" height=\"178\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-2-300x178.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-2-768x455.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-2.png 1011w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-1393\" class=\"wp-caption-text\">1:2 S-Trap Digestion: Mass spectrometer peaks from the APOA4 protein, SELTQQLNALFQDK peptide.<\/figcaption><\/figure>\n<figure id=\"attachment_1372\" aria-describedby=\"caption-attachment-1372\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1372 size-medium\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-in-soln-300x178.png\" alt=\"\" width=\"300\" height=\"178\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-in-soln-300x178.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-in-soln-768x457.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-in-soln.png 1021w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-1372\" class=\"wp-caption-text\">Traditional Digestion: Mass spectrometer peaks from the APOA4 protein, SELTQQLNALFQDK peptide.<\/figcaption><\/figure>\n<figure id=\"attachment_1373\" aria-describedby=\"caption-attachment-1373\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1373 size-medium\" src=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-1-300x178.png\" alt=\"\" width=\"300\" height=\"178\" srcset=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-1-300x178.png 300w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-1-768x456.png 768w, https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/split-1-v-1.png 1012w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-1373\" class=\"wp-caption-text\">1:1 S-Trap Digestion: Mass spectrometer peaks from the APOA4 protein, SELTQQLNALFQDK peptide.<\/figcaption><\/figure>\n<h3><strong>Conclusions:<\/strong><\/h3>\n<p>Data from the mass spectrometer led Shannon and Emily to the following conclusions:<\/p>\n<ul>\n<li>Based on the data collected, the S-Trap provides a sufficient alternative to in-solution digestion: retained peptides were at similar or higher levels than the traditional method. In particular, the S-Trap method with a binding buffer to sample ratio of 1:1 had the best peptide retention in this trial.<\/li>\n<li>The S-Trap protocol can be applied to larger volume depleted plasma samples without interfering with efficiency and accuracy.<\/li>\n<li>Further experimentation (repeated trials) will be necessary to confirm these results, but the S-Trap has strong potential and viability for use with ISB&#8217;s Lyme Disease research.<\/li>\n<\/ul>\n<h3><strong>For more information:<\/strong><\/h3>\n<p>On the S-Trap project: <a href=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-content\/uploads\/sites\/6\/2018\/08\/S-Trap-Poster.pdf\">Click here<\/a><\/p>\n<p>On the ISB Lyme project: <a href=\"https:\/\/systemsbiology.org\/research\/wilke-lyme-disease-project\/\">Click here<\/a><\/p>\n<p>On Lyme disease: <a href=\"https:\/\/www.cdc.gov\/lyme\/index.html\">Click here<\/a><\/p>\n<p>Contact us:\u00a0e.huynh247@gmail.com, shannonchauusa@yahoo.com<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Emily Huynh\u00a0&amp;\u00a0Shannon Chau Overview of Lyme Disease and the Lyme Project Lyme disease is a multi-organ illness that is transmitted to humans via ticks. Ticks act as a vector for the disease-causing spirochete Borrelia burgdoferi. Lyme disease was traditionally prevalent in the Northeast region, but&nbsp;<a class=\"read-more\" href=\"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/lyme-project\/\">&hellip;<\/a><\/p>\n","protected":false},"author":28,"featured_media":544,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-526","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/pages\/526","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/users\/28"}],"replies":[{"embeddable":true,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/comments?post=526"}],"version-history":[{"count":75,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/pages\/526\/revisions"}],"predecessor-version":[{"id":1635,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/pages\/526\/revisions\/1635"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/media\/544"}],"wp:attachment":[{"href":"https:\/\/baliga.systemsbiology.net\/see-interns\/hs2018\/wp-json\/wp\/v2\/media?parent=526"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}