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Unit 1 Ecological networks - Part 1 Network interactions
Laboratory Equipment Requirement: 
| Safety Equipment |
Materials per Group | Equipment per Group | Class Equipment |
|---|---|---|---|
| gloves * | Deionized water | Spectrophotomer | Shaker incubator**** |
| goggles* | 2.3M, 2.8M, 3.3M, 3.8M, 4.3M Growth Medium (GM) | 4 cuvettes | |
| apron* | Live Halobacterium culture | Large rack for Falcon tubes | |
| Falcon tube labels | Kim wipes | ||
| Beral pipet | |||
| P200 micropipet w/tips ** | |||
| 400-1000mL beaker for waste | |||
| 4 Falcon tubes, 17x100mm*** |
* no harmful materials - these materials are recommended but not required.
**Beral pipet may be used. 100μL only fills the first 2 segments (about 4 drops). Discussion of error/precision will be necessary.
***Falcon tubes are provided in the kit for use if you have a shaker incubator. If not, these tubes may be set inside an incubator and periodically shaken (gently). Add an additional 24 hours before checking the OD.
****If you do not have a shaker incubator, you may use small flasks and stir plates. See stir plate protocol within this module - Lesson 6.
To make your own Growth Media - please follow this recipe for optimal results.
Advance preparation and materials required for each lesson:
| Lesson | Description | Preparation and materials |
| Lesson 1 | Building cell phone network manually on paper | Lesson 1 Prep.doc |
| Lesson 2 | Cytoscape analysis of cell phone network | Lesson 2 Prep.doc |
| Lesson 3 | Researching background on extremophiles | Lesson 3 Prep.doc |
| Lesson 4 | Introduction to the GSL case study; hypothesis generation and experimental design | Lesson 4 Prep.doc |
| Lesson 5 | Practicing use of spectrophotometer and micropipettes | Lesson 5 Prep.doc |
| Lesson 6 | Conducting halobacteria salinity investigation | Lesson 6 Prep.doc |
| Lesson 7 | Analysis of halobacteria salinity data: *Assessment- writing a conclusion *Assessment- designing an investigation |
Lesson 7 Prep.doc |
| Lesson 8 | Incorporating laboratory data into GSL context to explain observed phenomena | Lesson 8 Prep.doc |